Journal: iScience
Article Title: Astrocytic FABP5 mediates retrograde endocannabinoid transport at central synapses
doi: 10.1016/j.isci.2025.112342
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Article Snippet: The following AAVs were used: AAV-CAG-FABP5 expressing mouse FABP5 under the CAG promoter (Duke VVC #pBK366, serotype AAV9), AAV-CAG-FABP5 MUT expressing FABP5 MUT (Duke VVC #1937, serotype AAV9), AAV-CAG-FABP5 SEC expressing FABP5 SEC (Duke VVC #pBK383, serotype AAV9), AAV-CAG-FABP7 expressing mouse FABP7 (Duke VVC #pBK1433, serotype AAV9), AAV-CMV-Cre (Addgene, #105545, serotype AAV9), AAV-CMV-GFP (Addgene, #105530, serotype AAV9), AAV-hSyn-Cre (Addgene, #105540, serotype AAV5), pAAV-hSyn-EGFP (Addgene, #50465, serotype AAV5), AAV-gfaABC1D-FABP5 expressing FABP5 under the astrocyte-specific gfaABC1D promoter (Duke VVC #pBK1762, serotype AAV8), pAAV.GFAP.eGFP.WPRE.hGH (Addgene, #105549, serotype AAV5), and AAV-hSyn-FABP5 (Duke VVC #pBK1761, serotype AAV5).
Techniques: Virus, Recombinant, Software, Imaging, Transfection, Real-time Polymerase Chain Reaction, SYBR Green Assay, Mass Spectrometry, Control, Microscopy